Upload your OTU/ASV table

[For detailed information on how the files have to look, check out the Info & Settings tab on the left!]


Upload your MSD data directly into Namco


You can either upload a file with mulitple links from MSD and then choose one or paste a single link directly into the corresponding field.


If you choose to work with zOTUs, the phylogenetic tree has to be built first. This leads to longer upload times.

Upload fastq sequencing files

[For detailed information on file specifications, check out the Info & Settings tab on the left!]

For details on the sample-name cutoff feature, check out the sample-names explanation in the 'Info & Settings' tab!


Explore quality profiles of provided fastq files


Select one of the following two amplicon sequencing analysis pipelines to process the fastq files:

Primer trimming in DADA2 is position based, so you need to know the length of your used primers. It is also assumed that primer sequences are at the beginning (left) of each read. If primers are already removed from your reads, enter 0.

The quality profiles above can guide you to find more fitting cutoff values


Primer trimming in LotuS2 is sequence based, so you need to know the sequence of your used primers. The tool will search for this sequence in each read and remove it.

Lotus2 will build a phylogenetic tree by default. If you do not need it, simply insert -buildPhylo 0 in the text field above.

Upload data from MSD

Explain MSD + link



Start by uploading your data or use our provided sample dataset and try out all the features in NAMCO


Documentation

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Overview of NAMCO

Data Overview & Filtering

Please consider filtering your data!

fastq Overview

Basic Analysis

Differential Analysis

Functional Analysis

Phylogenetic Analysis

Network Analysis

Confounding Analysis

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Information & global settings